That question really depends on a number of things, such as how you plan to layout your store listings (tables vs. CSS) and your ability to create the images that you want to have. Designing for Ebay is really no different than designing any other web page. If you have Adobe/Macromedia Fireworks, or even photoshop (though it may not be as easily done in PS) you can create your framework, slice it up, and export it as html and to be opened in dreamweaver for the final touch-ups.
I’ve done one Ebay store and it really wasn’t any different than creating any other static html page, you just have to make sure your image paths are correct (they’ll get changed in dreamweaver/fireworks). If you’re familiar with this, all you really need to do is create your store shell using these tools, copy your html in to Ebay and then use it as a profile/preset for your store template. We use Blackthorne to manage our store and it stores the html/profile info so all we have to do is create the individual products and apply the theme. I think there may be a similar feature in Turbo Lister, but I’m not sure.